Notice of retraction
Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

Instructions to authors
English    日本語

Instructions for manuscript preparation
English    日本語


 Sensors and Materials
 1-23-3-303 Sendagi,
 Bunkyo-ku, Tokyo 113-0022, Japan
 Tel: 81-3-3827-8549
 Fax: 81-3-3827-8547

MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.

MYU Research

(proofreading and recording)

(translation service)

The Art of Writing Scientific Papers

(How to write scientific papers)
(Japanese Only)

Sensors and Materials, Volume 19, Number 5 (2007)
Copyright(C) MYU K.K.
pp. 261-273
S&M681 Research Paper of Special Issue
Published: 2007

Novel DNP-KLH Protein Conjugate Surface for Sensitive Detection of TNT on SPR Immunosensor [PDF]

Praveen Singh, Takeshi Onodera, Yutaka Mizuta, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko

(Received March 28, 2007; Accepted May 1, 2007)

Keywords: TNT sensor, SPR sensor, inhibition assay, DNP-KLH protein conjugate

A nanoscale biosensor chip surface for the detection of trinitrotoluene (TNT) was fabricated using dinitrophenylated-keyhole lympet hemocyanin (DNP-KLH) protein conjugate as ligand supported by an underlying 11-amino 1-undecanethiol hydrochloride (AUT) self-assembled monolayer (SAM) and bis sulfo-succinimidyl suberate (BS3) as crosslinker. Bioactive thin films were fabricated over gold chip via layer-by-layer self-assembly methods. The biomolecular interaction between substrate-specific TNP-glycine-KLH mouse Ig antibody and DNP-KLH conjugate surface was monitored using a surface plasmon resonance-based optical sensor. The quantitation of (TNT) on this bioactive surface was carried out using the solution-based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.15 ng/ml (150 ppt) and was sensitive up to the 0.005 ng/ml (5 ppt) level for the TNT molecule. This TNT-specific biosensor holds the promise to be one of the most sensitive, fast TNT detecting sensor surfaces under indirect competitive assay format. A 12 s injection pulse of 10 mM glycine-HCl solution was sufficient for the regeneration of the DNP-KLH surface for repeated use. The DNP-KLH sensor platform was checked for its reproducibility and storability.

Corresponding author: Praveen Singh

Cite this article
Praveen Singh, Takeshi Onodera, Yutaka Mizuta, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko, Novel DNP-KLH Protein Conjugate Surface for Sensitive Detection of TNT on SPR Immunosensor, Sens. Mater., Vol. 19, No. 5, 2007, p. 261-273.

Forthcoming Regular Issues

Forthcoming Special Issues

Special Issue on Perceptual Deep Learning in Computer Vision and its Application
Guest editor, Chih-Hsien Hsia (National Ilan University)

Special issue on Novel Materials and Sensing Technologies on Electronic and Mechanical Devices (1)
Guest editor, Teen-Hang Meen (National Formosa University), Wenbing Zhao (Cleveland State University), and Hsien-Wei Tseng (Longyan University)
Call for paper

Special Issue on New Trends in Smart Sensor Systems
Guest editor, Takahiro Hayashi (Kansai University)
Call for paper

Special Issue on International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS and Applications 2019 (Bio4Apps 2019)
Guest editor, Hirofumi Nogami and Masaya Miyazaki (Kyushu University)
Conference website
Call for paper

Special Issue on Materials, Devices, Circuits, and Analytical Methods for Various Sensors (4)
Guest editor, Chien-Jung Huang (National University of Kaohsiung), Cheng-Hsing Hsu (National United University), Ja-Hao Chen (Feng Chia University), and Wei-Ling Hsu (Huaiyin Normal University)
Conference website

Special Issue on Geomatics Technologies for the Realization of Smart Cities
Guest editor, He Huang and XiangLei Liu (Beijing University of Civil Engineering and Architecture)
Call for paper

Copyright(C) MYU K.K. All Rights Reserved.