ISSN 0914-4935
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

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Real-time Polymerase Chain Reaction System Using a Camera for Open Platform

Ji-Soo Hwang, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, Jong-won Kim, and Jong-Dae Kim

(Received April 16, 2018; Accepted March 20, 2019)

Keywords: fluorescence detection, USB camera, real-time polymerase chain reaction, open platform

In real-time polymerase chain reaction (PCR), the result of the amplification of DNA can be seen through changes in fluorescence brightness sensed during the PCR cycles. To detect the changes in fluorescence intensities, commercial real-time PCR thermocyclers mainly use photodiodes for detection. However, our previous study showed that a digital single lens reflex (DSLR) camera instead of a photodiode can detect changes in fluorescence intensity as well as differentiate cases of unsatisfactory experimental results. Although the fluorescence detection was successful, the adoption of the DSLR camera was problematic in terms of size and cost. In this paper, we show that fluorescence detection is also possible using a small USB camera for open platforms instead of bulky DSLR cameras.

Corresponding author: Jong-Dae Kim




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