S&M Young Researcher Paper Award 2020
Recipients: Ding Jiao, Zao Ni, Jiachou Wang, and Xinxin Li [Winner's comments]
Paper: High Fill Factor Array of Piezoelectric Micromachined
Ultrasonic Transducers with Large Quality Factor

S&M Young Researcher Paper Award 2021
Award Criteria
Notice of retraction
Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

Instructions to authors
English    日本語

Instructions for manuscript preparation
English    日本語

Template
English

Publisher
 MYU K.K.
 Sensors and Materials
 1-23-3-303 Sendagi,
 Bunkyo-ku, Tokyo 113-0022, Japan
 Tel: 81-3-3827-8549
 Fax: 81-3-3827-8547

MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.


MYU Research

(proofreading and recording)


MYU K.K.
(translation service)


The Art of Writing Scientific Papers

(How to write scientific papers)
(Japanese Only)

Sensors and Materials, Volume 31, Number 1(1) (2019)
Copyright(C) MYU K.K.
pp. 1-11
S&M1742 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2019.2032
Published: January 15, 2019

Fingerprint-based Protein Identification in Cell Culture Medium Using Environment-sensitive Turn-on Fluorescent Polymer [PDF]

Hiroka Sugai, Shunsuke Tomita, Sayaka Ishihara, and Ryoji Kurita

(Received June 25, 2018; Accepted August 24, 2018)

Keywords: proteins, biosensors, multivariate analysis, polymers, cell culture medium

The identification of secreted proteins in cell culture supernatants is a useful method for the noninvasive evaluation of cultured cells. Herein, we show that a fingerprint-based sensor technique can be used to identify typical hepatocyte-derived secretory proteins spiked into a cell culture medium. A poly-L-lysine modified with environment-sensitive dansyl groups (PLL-Dnc), which allows a turn-on fluorescent response with cross-reactivity against different analytes, was employed for the sensing of a series of secretory proteins (albumin, α1-antitrypsin, fibrinogen, transferrin, and α-fetoprotein). An array of PLL-Dnc in different buffer solutions successfully produced fluorescence fingerprints as a result of distinct interactions with analyte proteins depending on solution conditions (pH and ionic strength), enabling the qualitative identification of five secretory proteins in culture media (40 μg/mL) with 100% accuracy using linear discriminant analysis. The array system was also capable of analyzing culture media that contain different concentrations of albumin and α-fetoprotein under realistic conditions. This work demonstrates the solution-condition-dependent discriminatory response of PLL-Dnc toward proteins spiked into a culture medium, rendering such a PLL-Dnc system a promising platform for the antibody-free and marker-based evaluation of cultured cells.

Corresponding author: Shunsuke Tomita


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Cite this article
Hiroka Sugai, Shunsuke Tomita, Sayaka Ishihara, and Ryoji Kurita, Fingerprint-based Protein Identification in Cell Culture Medium Using Environment-sensitive Turn-on Fluorescent Polymer, Sens. Mater., Vol. 31, No. 1, 2019, p. 1-11.



Forthcoming Regular Issues


Forthcoming Special Issues

Special Issue on Advanced Materials and Sensing Technologies on IoT Applications: Part 1-2
Guest editor, Teen-Hang Meen (National Formosa University), Wenbing Zhao (Cleveland State University), and Cheng-Fu Yang (National University of Kaohsiung)


Special Issue on Smart Sensing Technologies and Their Application in Forest Management and Engineering
Guest editor, Byoungkoo Choi (Kangwon National University)
Call for paper


Special Issue on Advanced Materials and Sensing Technologies on IoT Applications: Part 2-1
Guest editor, Teen-Hang Meen (National Formosa University), Wenbing Zhao (Cleveland State University), and Cheng-Fu Yang (National University of Kaohsiung)
Call for paper


Special Issue on International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS and Applications 2019 (Bio4Apps 2019) (2)
Guest editor, Hirofumi Nogami and Masaya Miyazaki (Kyushu University)
Conference website


Special Issue on High-sensitivity Sensors and Sensors for Difficult-to-measure Objects
Guest editor, Ki Ando (Chiba Institute of Technology)
Call for paper


Special Issue on Biological Odor Sensing System and Their Applications
Guest editor, Takeshi Sakurai (Tokyo University of Agriculture)
Call for paper


Copyright(C) MYU K.K. All Rights Reserved.