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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials
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Sensors and Materials, Volume 27, Number 9 (2015)
Copyright(C) MYU K.K.
pp. 839-845
S&M1120 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2015.1176
Published: October 7, 2015

Development of an Electrochemical Adenosine Diphosphate (ADP) Sensing Principle Employing ADP-dependent Kinase [PDF]

Wakako Tsugawa, Hinako Futagami and Koji Sode

(Received February 18, 2015; Accepted August 18, 2015)

Keywords: ADP-dependent kinase, screen-printed carbon electrode, ADP-dependent hexokinase, electrochemical assay, ATP-dependent kinase

In this study, a novel electrochemical sensing principle was developed for adenosine diphosphate (ADP), which utilizes ADP-dependent kinase as the key enzyme. In this principle, ADP-dependent hexokinase, glucose-6-phosphate dehydrogenase, and diaphorase are combined in the presence of glucose, nicotinamide adenine dinucleotide, and hexaammineruthenium (III) as the electron mediator. The reaction was conducted in a homogeneous mixture on a disposable screen-printed carbon electrode. The measurement was completed within 80 s, and ADP was measured between 2 and 50 μM with a detection limit of 1.9 μM (S/N = 3). This simple, rapid method can be generally applicable as an electrochemical assay for the detection of adenosine-triphosphate-dependent kinases.

Corresponding author: Koji Sode


Cite this article
Wakako Tsugawa, Hinako Futagami and Koji Sode, Development of an Electrochemical Adenosine Diphosphate (ADP) Sensing Principle Employing ADP-dependent Kinase, Sens. Mater., Vol. 27, No. 9, 2015, p. 839-845.



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