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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 27, Number 9 (2015)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 839-845
S&M1120 Research Paper of Special Issue https://doi.org/10.18494/SAM.2015.1176 Published: October 7, 2015 Development of an Electrochemical Adenosine Diphosphate (ADP) Sensing Principle Employing ADP-dependent Kinase [PDF] Wakako Tsugawa, Hinako Futagami and Koji Sode (Received February 18, 2015; Accepted August 18, 2015) Keywords: ADP-dependent kinase, screen-printed carbon electrode, ADP-dependent hexokinase, electrochemical assay, ATP-dependent kinase
In this study, a novel electrochemical sensing principle was developed for adenosine diphosphate (ADP), which utilizes ADP-dependent kinase as the key enzyme. In this principle, ADP-dependent hexokinase, glucose-6-phosphate dehydrogenase, and diaphorase are combined in the presence of glucose, nicotinamide adenine dinucleotide, and hexaammineruthenium (III) as the electron mediator. The reaction was conducted in a homogeneous mixture on a disposable screen-printed carbon electrode. The measurement was completed within 80 s, and ADP was measured between 2 and 50 μM with a detection limit of 1.9 μM (S/N = 3). This simple, rapid method can be generally applicable as an electrochemical assay for the detection of adenosine-triphosphate-dependent kinases.
Corresponding author: Koji Sode Cite this article Wakako Tsugawa, Hinako Futagami and Koji Sode, Development of an Electrochemical Adenosine Diphosphate (ADP) Sensing Principle Employing ADP-dependent Kinase, Sens. Mater., Vol. 27, No. 9, 2015, p. 839-845. |
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