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Sensors and Materials, Volume 27, Number 9 (2015)
Copyright(C) MYU K.K.
pp. 825-838
S&M1119 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2015.1146
Published: October 7, 2015

Establishment of Ferricyanide Chronoamperometric Total Antioxidant Capacity Assay Employing a Carbon Screen-Printed Disposable Microchip —Fundamental Study Using Vegetable Extraction— [PDF]

Masao Gotoh, Hideo Hirose, Tomoko Ishikawa, Hideaki Nakamura and Kenji Yokoyama

(Received February 3, 2015; Accepted June 17, 2015)

Keywords: microchip, antioxidant, methanol, solubility valance, vegetable

In the present study, a carbon screen-printed disposable microchip as a microstructured two-electrode system was developed for the establishment of a ferricyanide chronoamperometric total antioxidant capacity (FC-TAC) assay for vegetables. Ferricyanide cyclic voltammetry (FCV) measurements were also performed using the microchip to define optimum conditions for the FC-TAC assay. As antioxidants, we chose ascorbic acid (vitamin C), chlorogenic acid, and α-tocopherol (vitamin E). The electrochemical response to an antioxidant was indirectly determined through ferricyanide and was strongly affected by the solubility of both the mediator and the antioxidant in the measurement solution. By intensive investigation, it was found that 80% (v/v) methanol was the best solvent for the FC measurements and vegetable extraction. Under optimum conditions for the FC-TAC assay, micro-volume measurement of 30 µL without reaction time was realized using the microchip, and calibration curves for three antioxidants were successfully obtained with excellent determination coefficients (r2 > 0.986, n = 5). Finally, the conventional DPPH test using ten vegetable extractions was applied for comparison with the present FC-TAC method, and the results obtained showed good correspondence (r2 > 0.928, n = 5).

Corresponding author: Masao Gotoh


Cite this article
Masao Gotoh, Hideo Hirose, Tomoko Ishikawa, Hideaki Nakamura and Kenji Yokoyama, Establishment of Ferricyanide Chronoamperometric Total Antioxidant Capacity Assay Employing a Carbon Screen-Printed Disposable Microchip —Fundamental Study Using Vegetable Extraction—, Sens. Mater., Vol. 27, No. 9, 2015, p. 825-838.



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