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Vol. 34, No. 8(3), S&M3042

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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
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Sensors and Materials, Volume 16, Number 8 (2004)
Copyright(C) MYU K.K.
pp. 391-399
S&M576 Research Paper of Special Issue
Published: 2004

Preparation of Phosphate-Binding-Protein-Modified Electrode and Its Application to Reagentless Phosphate Sensor [PDF]

Izumi Kubo and Daisuke Satoh

(Received July 2, 2004; Accepted November 17, 2004)

Keywords: potentiometric, phosphate binding protein (PBP), gold electrode, reagentless

To develop a simple and reagentless phosphate sensing system, phosphate binding protein (PBP) was utilized as a recognition element for the sensor. The preparation of a PBP-modified electrode was investigated. PBP was immobilized on a gold electrode surface through cysteamine and glutaraldehyde. The amount of immobilized PBP was 13.7 ng/mm2, which corresponded to three layers of PBP. The response of the PBP- modified electrode to phosphate was measured as the potential change of the electrode and the response time was about 2 min. The response to phosphate was –0.505 mV/mM in the concentration range of 10–50 mM of phosphate. To evaluate the nonspecific response to ions other than phosphate, a malic-dehydrogenase-immobilized electrode was compared with the PBP-modified electrode. The response to phosphate of the PBP-modified electrode was specific but those to other ions were almost same as those of the malic- dehydrogenase-modified electrode.

Corresponding author: Izumi Kubo


Cite this article
Izumi Kubo and Daisuke Satoh, Preparation of Phosphate-Binding-Protein-Modified Electrode and Its Application to Reagentless Phosphate Sensor, Sens. Mater., Vol. 16, No. 8, 2004, p. 391-399.



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