pp. 4797-4808
S&M3432 Research Paper of Special Issue https://doi.org/10.18494/SAM4570 Published: October 25, 2023 C-reactive Protein Detection Using an Ion-sensitive Field-effect Transistor (ISFET)-based Aptasensor with a Chemically Modified Gate Surface for Improved Sensitivity [PDF] Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Masayoshi Esashi, and Hitoshi Shiku (Received June 30, 2023; Accepted October 12, 2023) Keywords: aptamer, ion-sensitive field-effect transistor (ISFET), C-reactive protein, silane coupling, pH sensitivity
C-reactive protein (CRP) is an inflammation biomarker that requires simple and real-time monitoring for accurate diagnosis. Conventional CRP tests are complicated, expensive, and time-consuming. Field-effect transistor (FET)-based affinity sensors are seen as the ideal solution but it is difficult to obtain FET with sensitive gate structures. In this work, we propose a simple method of chemically modifying the gate surface of a commercial ion-sensitive FET (ISFET) with (3-glycidyloxypropyl)trimethoxysilane (GPTMS) to lower the background noise signal and then immobilize aptamers that provide significant surface potential change when they bind to CRP. The FET aptasensor was able to measure 0.002–20 μg/mL CRP in 1×PBS with a higher sensitivity than the nonmodified ISFET sensors with their original pH sensitivity and was on par with other FET sensors without needing expensive nanomaterial or complicated nanofabrication.
Corresponding author: Kumi Y. InoueThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Masayoshi Esashi, and Hitoshi Shiku, C-reactive Protein Detection Using an Ion-sensitive Field-effect Transistor (ISFET)-based Aptasensor with a Chemically Modified Gate Surface for Improved Sensitivity, Sens. Mater., Vol. 35, No. 10, 2023, p. 4797-4808. |