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Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials, Volume 23, Number 1 (2011)
Copyright(C) MYU K.K.
pp. 39-52
S&M825 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2011.711
Published: January 28, 2011

Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene [PDF]

Takeshi Onodera, Yutaka Mizuta, Kazuhiko Horikawa, Praveen Singh, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko

(Received June 1, 2010; Accepted August 24, 2010)

Keywords: surface plasmon resonance, displacement, TNT, self-assembled monolayer, antigen-antibody interaction

By making use of the antigen-antibody interaction and a surface plasmon resonance (SPR) sensor, we developed a measurement procedure of displacement immunoassay to rapidly detect 2,4,6-trinitrotoluene (TNT). In this procedure, TNT solutions were injected in 30 s at the end of flowing an anti-TNT antibody. Three kinds of sensor surfaces were modified with TNT analogues, namely, TNP-glycine, DNP-glycine, and DNP-acetic acid in a self-assembled monolayer containing ethylene glycol. We investigated which of the TNT analogues provided higher sensitivity using the displacement immunosensor. As a result, the limit of detection (LOD) of TNT was 0.4 ng/mL (ppb) when using the DNP-glycine-modified Au sensor surface with a one-minute flow of TNT solution. We concluded that the final TNT LOD was 0.9 ppb on the basis of experiments using the three different DNP-glycine-modified sensor surfaces. The LOD was 0.7 ppb when using the sensorgram slope 10 s after TNT injection. The displacement immunosensor can detect TNT at sub-ppb levels in 12 s.

Corresponding author: Takeshi Onodera


Cite this article
Takeshi Onodera, Yutaka Mizuta, Kazuhiko Horikawa, Praveen Singh, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko, Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene, Sens. Mater., Vol. 23, No. 1, 2011, p. 39-52.



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