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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 23, Number 1 (2011)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 39-52
S&M825 Research Paper of Special Issue https://doi.org/10.18494/SAM.2011.711 Published: January 28, 2011 Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene [PDF] Takeshi Onodera, Yutaka Mizuta, Kazuhiko Horikawa, Praveen Singh, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko (Received June 1, 2010; Accepted August 24, 2010) Keywords: surface plasmon resonance, displacement, TNT, self-assembled monolayer, antigen-antibody interaction
By making use of the antigen-antibody interaction and a surface plasmon resonance (SPR) sensor, we developed a measurement procedure of displacement immunoassay to rapidly detect 2,4,6-trinitrotoluene (TNT). In this procedure, TNT solutions were injected in 30 s at the end of flowing an anti-TNT antibody. Three kinds of sensor surfaces were modified with TNT analogues, namely, TNP-glycine, DNP-glycine, and DNP-acetic acid in a self-assembled monolayer containing ethylene glycol. We investigated which of the TNT analogues provided higher sensitivity using the displacement immunosensor. As a result, the limit of detection (LOD) of TNT was 0.4 ng/mL (ppb) when using the DNP-glycine-modified Au sensor surface with a one-minute flow of TNT solution. We concluded that the final TNT LOD was 0.9 ppb on the basis of experiments using the three different DNP-glycine-modified sensor surfaces. The LOD was 0.7 ppb when using the sensorgram slope 10 s after TNT injection. The displacement immunosensor can detect TNT at sub-ppb levels in 12 s.
Corresponding author: Takeshi Onodera Cite this article Takeshi Onodera, Yutaka Mizuta, Kazuhiko Horikawa, Praveen Singh, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko, Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene, Sens. Mater., Vol. 23, No. 1, 2011, p. 39-52. |
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