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Recipients: Ding Jiao, Zao Ni, Jiachou Wang, and Xinxin Li [Winner's comments]
Paper: High Fill Factor Array of Piezoelectric Micromachined
Ultrasonic Transducers with Large Quality Factor

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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials, Volume 32, Number 12(2) (2020)
Copyright(C) MYU K.K.
pp. 4131-4138
S&M2399 Research Paper of Special Issue (Bio4Apps 2019)
https://doi.org/10.18494/SAM.2020.2897
Published: December 16, 2020

Portable Electrochemical Immunochromatography Platform for Point-of-care Testing and Comparison with Enzyme-linked Immunosorbent Assay [PDF]

Wataru Iwasaki, Nobutomo Morita, and Masaya Miyazaki

(Received April 2, 2020; Accepted June 17, 2020)

Keywords: electrochemical measurement, immunochromatography, paper-based analytical device, point-of-care testing

We conducted electrochemical immunochromatography using a developed electrochemical immunochromatography platform. We used immunoglobulin G (IgG) as a representative protein. We also conducted enzyme-linked immunosorbent assay (ELISA) as a control test. We used the same reagents except for the substrate and compared their limits of detection (LODs) and detection ranges. We succeeded in measuring IgG concentrations ranging from 30 ng/ml to 10 µg/ml and obtained 23.00 ng/ml as the LOD for electrochemical immunochromatography. The detection signal was not saturated even at 10 µg/ml, so it seems that higher concentrations of IgG can also be measured. The LOD was approximately 80-fold higher than that of ELISA (0.30 ng/ml); however, the detection range was one order of magnitude higher than that of ELISA. While electrochemical immunochromatography is less sensitive than ELISA, we demonstrated sufficient sensitivity for its use in a point-of-care testing (POCT) device. Furthermore, this information will be very helpful when introducing an assay system already established in ELISA to POCT.

Corresponding author: Wataru Iwasaki


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Cite this article
Wataru Iwasaki, Nobutomo Morita, and Masaya Miyazaki, Portable Electrochemical Immunochromatography Platform for Point-of-care Testing and Comparison with Enzyme-linked Immunosorbent Assay, Sens. Mater., Vol. 32, No. 12, 2020, p. 4131-4138.



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