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Sensors and Materials, Volume 32, Number 8(1) (2020)
Copyright(C) MYU K.K.
pp. 2585-2596
S&M2285 Research Paper of Special Issue (Prof. Yang)
Published: August 10, 2020

Motility-driven Sperm-sorting Microfluidic Chip with Little Cell Damage for Oligozoospermia Patients [PDF]

Hong-Yuan Huang, Cheng-Ying Lu, I-Wen Wang, and Da-Jeng Yao

(Received September 30, 2019; Accepted April 8, 2020)

Keywords: microfluidic chip, sperm sorting, laminar flow, artificial reproduction, oligozoospermia

Various assisted reproductive techniques (ARTs) are now available for infertile couples. Obtaining a large amount of highly active sperm is critical for successful ART operations. Our purpose is to use polydimethylsiloxane (PDMS) microfluid biochip to collect highly active sperm from semen with the characteristic of laminar flow. With a fixed diluted semen viscosity and a fixed flow rate ratio of buffer and diluted semen, this microfluid biochip can sort diluted semen into two groups: sorted and waste. Results of flow cytometry analysis showed that the viability of the sorted group is higher by at least 10% for both normal and oligozoospermia samples. Our biochip, compared with the traditional centrifuge, is a novel tool for preparing live sperm for ARTs.

Corresponding author: Da-Jeng Yao

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Cite this article
Hong-Yuan Huang, Cheng-Ying Lu, I-Wen Wang, and Da-Jeng Yao, Motility-driven Sperm-sorting Microfluidic Chip with Little Cell Damage for Oligozoospermia Patients, Sens. Mater., Vol. 32, No. 8, 2020, p. 2585-2596.

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