ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

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Nonlabeling and Nonexternal Indicator DNA Sensing Based on Ferrocene-terminated Probes Immobilized on Gold Film Electrode Arrays Treated with Plasma and Acid Exposures

Hiroshi Aoki, Takeshi Sukegawa, Masaki Torimura, and Tetsuya Nakazato

(Received September 30, 2019; Accepted January 27, 2020)

Keywords: oligonucleotides, DNAs, sensor array chips, long-term storage, activation

We fabricated a novel sensor array chip that enables simple, rapid, and sequence-specific DNA detection without any process to label DNAs or any external electrochemical indicators. We found the conditions for the cleaning of electrode surfaces and the preparation of DNA sensor array chips. A long-term storage for several weeks inactivated photolithographically fabricated 384-ch microelectrode array chips before surface modification. We revealed that the treatments with O2 plasma and acidic solutions reactivated the long-term stored chips, and demonstrated that the DNA sensor arrays prepared from the reactivated chips detected DNAs sequence-specifically, similarly to freshly fabricated sensors. We found that the feasible DNA sensor array chips can be prepared from the electrode array chips stored even for a long period, as long as the stored chips are treated before use with O2 plasma and acid solutions under optimized conditions.

Corresponding author: Hiroshi Aoki




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