Preparation of Biodegradable Polycaprolactone Microcarriers with Doxorubicin Hydrochloride by Ultrasonic-assisted Emulsification Technology
Yeong-Maw Hwang, Cheng-Tang Pan, Yu-Min Lin, Song-Wei Zeng, Chung-Kun Yen, Shao-Yu Wang, Shiao-Wei Kuo, Shin-Pon Ju, Shih-Shin Liang, and Zong-Hsin Liu
(Received May 31, 2018; Accepted October 11, 2018)
Keywords: ultrasonic spray, emulsion-solvent evaporation process, polycaprolactone, biodegradable microcarrier, Doxorubicin
Biomedical materials should have biodegradability (enzymatic degradation and hydrolysis) because biodegradable materials in clinical applications show excellent biocompatibility. Polycaprolactone (PCL) is type of semi crystalline polymer that can be utilized in various organic solvents. Therefore, PCL was made as controlled-release drug carriers for long-term treatment. In is study, we propose how to make biodegradable PCL microcarriers with a uniform and controllable particle size (size range is ~ 5 - 100 µm) by emulsion-solvent evaporation with ultrasonic micro-droplet spray technology. The ultrasonic spray was assisted the emulsion-solvent evaporation. Through the standing wave effect on the ultrasonic nozzle, the continuous oil solution of PCL became discontinuous, and the micro droplets of the oil solution were sprayed out directly with high uniformity particle size. Furthermore, the oil micro droplets of PCL were collected by an aqueous stream in the emulsion process to form the emulsion type of oil in water (O/W). In addition, the ultrasonic assisting emulsification technology was adjusted by stirring to enhance the uniformity of the particle size. As a result, PCL micro droplets were sprayed into an aqueous solution owing to the low surface tension between the oil and the aqueous solution and the vertical flow field made by the stirring equipment. The yield rate of the PCL microcarriers was improved effectively. Moreover, microcarriers with the cancer drug Doxorubicin (DOX), which is water-soluble, were fabricated uniformly with ultrasonic-assisted emulsification technology. The drug loading efficiency in this work was ~ 42.2%, and the encapsulation efficiency of microcarriers of 3.21% was obtained with a particle size of around 5-20 µm.
Corresponding author: Cheng-Tang Pan