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Sensors and Materials
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Sensors and Materials, Volume 30, Number 12(3) (2018)
Copyright(C) MYU K.K.
pp. 2989-2998
S&M1735 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2018.2069
Published: December 28, 2018

Fabrication of Graphene Microroll Aptasensor [PDF]

Yuko Ueno, Tetsuhiko Teshima, Calum Henderson, and Hiroshi Nakashima

(Received July 31, 2018; Accepted September 12, 2018)

Keywords: graphene, aptamer, biosensor, self-folding, microdevice

We have successfully created a graphene microroll aptasensor (GMA) through the self-folding of bilayer thin films. The inner surface of the GMA consists of a functionalized graphene surface, which works as a fluorescence aptamer-based biosensor. This is achieved by combining previously reported two-dimensional (2D) graphene aptasensor technology, a novel self-folding process for flexible, multilayered polymeric films. The aptasensing portion is composed of a graphene surface, which is functionalized with a pyrene–aptamer–dye probe. The three components of the probe work as linkers to the graphene surface, a protein recognition part, and a fluorescence detection tag, respectively. In this paper, we discuss the specialized fabrication process required to produce the GMA. By confirming the diffusion of target molecules through the hollow space of the GMA, we also show the ability of this device to detect concentration changes in real time.

Corresponding author: Yuko Ueno


Cite this article
Yuko Ueno, Tetsuhiko Teshima, Calum Henderson, and Hiroshi Nakashima, Fabrication of Graphene Microroll Aptasensor, Sens. Mater., Vol. 30, No. 12, 2018, p. 2989-2998.



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