Sensors and Materials

ISSN 0914-4935
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.

Tweets by Journal_SandM Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

Instructions to authors
English 日本語

Instructions for manuscript preparation
English 日本語

Template
English

Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547

MYU Research
(proofreading and recording)

MYU K.K.
(translation service)

The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)

pp. 63-70
S&M1748
https://doi.org/10.18494/SAM.2019.2044
Published: January 15, 2019

Waveguide-mode Illumination for Enhanced Fluorescent Microscopy [PDF]

Masato Yasuura and Makoto Fujimaki

(Received July 2, 2018; Accepted August 27, 2018)

Keywords: near-field light, fluorescent assay, immunoassay, total internal reflection

In this research, we apply the near field excited by waveguide mode (WGM) to the illumination of a total internal reflection fluorescence (TIRF) microscope. By utilizing the electric field enhancement by WGM, we can replace the laser light source with a low-output light source such as an LED. By measuring the reflection spectra of the WGM chip whose surface was covered with cells, the effect of the cell on the reflection spectra was evaluated. On the basis of the evaluation, we designed an asymmetric trapezoidal prism to excite two fluorescent dyes with different excitation wavelengths on a single WGM chip. By using the trapezoidal prism with different base angles to enhance each wavelength, enhanced fluorescence observations using two fluorescent dyes with a simple optical system became possible. In this optical system, the observation of cultured cells, whose cytoskeleton and nuclei were separately stained with two fluorescent dyes, was carried out. It was confirmed that the fluorescence of each dye was effectively enhanced at both wavelengths.

Corresponding author: Makoto Fujimaki

This work is licensed under a Creative Commons Attribution 4.0 International License.

Cite this article
Masato Yasuura and Makoto Fujimaki, Waveguide-mode Illumination for Enhanced Fluorescent Microscopy, Sens. Mater., Vol. 31, No. 1, 2019, p. 63-70.