Sensors and Materials 30th Anniversary Award
Award Criteria
S&M Young Researcher Paper Award 2015
 Recipient: Toshihiko Noda
Paper: Magnetic-Thrust Flexible Electrode with Integrated Preamplifier for Neural Recording
Comment from Award Recipient: [English] / [日本語]
ISSN 0914-4935

Sensors and Materials
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Waveguide-mode Illumination for Enhanced Fluorescent Microscopy

Masato Yasuura and Makoto Fujimaki

(Received July 2, 2018; Accepted August 27, 2018)

Keywords: near-field light, fluorescent assay, immunoassay, total internal reflection

In this research, we apply the near field excited by waveguide mode (WGM) to the illumination of the total internal reflection fluorescence (TIRF) microscope. By utilizing the electric field enhancement by WGM, we can replace the laser light source with a low-output light source such as an LED. By measuring the reflection spectra of the WGM chip whose surface was covered with cells, the effect of the cell on the reflection spectra was evaluated. On the basis of the evaluation, we designed an asymmetric-trapezoidal prism to excite two fluorescent dyes with different excitation wavelengths on a single WGM chip. By using the trapezoidal prism with different base angles to enhance each wavelength, enhanced fluorescence observations using two fluorescent dyes with a simple optical system became possible. In this optical system, the observation of cultured cells, which separate the cytoskeleton and nuclei with two fluorescent dyes, was carried out. It was confirmed that each fluorescence was effectively enhanced in both wavelengths.

Corresponding author: Makoto Fujimaki

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