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Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials, Volume 30, Number 3(1) (2018)
Copyright(C) MYU K.K.
pp. 397-402
S&M1501 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2018.1760
Published: February 28, 2018

Comparison of Real-time Polymerase Chain Reaction Results by Different Illumination Methods [PDF]

Ji-Soo Hwang, Jong-Dae Kim, Yu-Seop Kim, Hye-Jeong Song, and Chan-Young Park

(Received July 30, 2017; Accepted November 9, 2017)

Keywords: polymerase chain reaction, fluorescence detection test, PCR chip, lab on a chip, illumination method, real-time PCR, quantitative analysis

In this paper, we introduce the diagonal illumination method and the vertical illumination method of the real-time polymerase chain reaction (PCR) system and compare the fluorescence intensity change patterns of the two methods using images captured with a digital single lens reflex (DSLR) camera during the PCR process. To overcome the problems of diagonal illumination, the PCR was performed by vertical illumination. The results of the PCR process with the proposed vertical illumination method were similar to those of the diagonal illumination method.

Corresponding author: Chan-Young Park


Cite this article
Ji-Soo Hwang, Jong-Dae Kim, Yu-Seop Kim, Hye-Jeong Song, and Chan-Young Park, Comparison of Real-time Polymerase Chain Reaction Results by Different Illumination Methods, Sens. Mater., Vol. 30, No. 3, 2018, p. 397-402.



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