Algae have the ability to convert solar energy into organic compounds through photosynthesis and store them as lipids. The final step is the conversion of biolipids into biodiesel, which is a way to reduce carbon dioxide emissions and which can be considered as an inexhaustible alternative energy source. The process of studying the effects of biomechatronics involves an ultrasonic transducer and light emitting diode (LED) lights, which affect algal lipids. In an optimum extraction experiment, 0.18 g lipid can be extracted per gram of Chlorella after 5 d. Experiments with ultrasound increased lipid extraction by 19.13% compared with controls. The results of this study can be used for increasing the amount of lipid production that can be extracted from Chlorella, contributing to the development of better cultivation techniques with adequate environmental protection.