Neurotransmitters and neuronal releasing molecules are not only the regulators of neuronal function but also the indicators of neuronal conditions. Glutamate and γ-amino butyric acid (GABA) play important roles in cerebellar differentiation and function. In the mature cortex, they are released from synapses and taken up by transporter molecules. We have developed enzyme-linked photoassay systems for glutamate, GABA, and adenosine triphosphate (ATP), and reported their release in the developing cerebellar cortex. Our systems showed slow transmitter release in the immature cerebellum, whereas it was hard to detect the fast synaptic release from mature neurons, because there were some limitations in time resolution and data depth derived from a charge-coupled device (CCD), and the enzyme-linked photodevice was sometimes unstable. In this study, we report the dynamic observation of neurotransmitter release in the developing cerebellar slices using improved photodevices and a high-speed 16-bit CCD. With this new system, the rapid measurement of transmitter release in a young-adult cerebellar cortex is possible. We suggest that these photoassay systems are useful for observing synaptic release in several diseases.