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Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
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Sensors and Materials, Volume 27, Number 9 (2015)
Copyright(C) MYU K.K.
pp. 805-815
S&M1117 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2015.1141
Published: October 7, 2015

Carbon Nanotube-Enhanced Enzyme Sensor for Real-Time Monitoring of Cholesterol Levels in Free-Swimming Fish [PDF]

Taogesi, Haiyun Wu, Masataka Murata, Huifeng Ren and Hideaki Endo

(Received January 29, 2015; Accepted May 11, 2015)

Keywords: sensor, fish, carbon nanotube, cholesterol, electrode

Temporal changes in the plasma total cholesterol concentration of fish are an indication of fish health, and a continuous in vivo monitoring method for total cholesterol is therefore desirable. An enzyme biosensor would be a good choice for continuous measurement. However, a biosensor as a foreign substance has a low sensitivity after a certain period of measurement owing to immune system attack. We introduced a highly sensitive carbon nanotube (CNT) to enhance sensor sensitivity for the real-time monitoring of total cholesterol in free-swimming fish (Oreochromis niloticus). The sensor was constructed using a Pt-Ir electrode that was dipped into a dispersed CNT solution and dried to adsorb the CNT onto the electrode. Cholesterol esterase, cholesterol oxidase, and a mediator were then immobilized on the sensor. The proposed sensor output current correlated well (R = 0.9992) with a cholestreyl oleate standard (10–300 mg dl−1). The dynamic range matched the range of cholesterol concentrations in fish (50–300 mg dl−1). A sensor with a mediator was not affected by changes in oxygen concentration. Changes in total cholesterol concentration could be continuously monitored in free-swimming fish using this sensor for 26 h.

Corresponding author: Hideaki Endo


Cite this article
Taogesi, Haiyun Wu, Masataka Murata, Huifeng Ren and Hideaki Endo, Carbon Nanotube-Enhanced Enzyme Sensor for Real-Time Monitoring of Cholesterol Levels in Free-Swimming Fish, Sens. Mater., Vol. 27, No. 9, 2015, p. 805-815.



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