Notice of retraction
Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
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Sensors and Materials, Volume 16, Number 8 (2004)
Copyright(C) MYU K.K.
pp. 413-420
S&M578 Research Paper of Special Issue
Published: 2004

Protein Dot Stamp Using Hydrophobin as Immobilization Carrier [PDF]

Shinya Ikeno, Géza R. Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi and Tetsuya Haruyama

(Received July 29, 2004; Accepted November 17, 2004)

Keywords: hydrophobin, protein array, high throughput, dot spot

Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conven- tional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

Corresponding author: Tetsuya Haruyama


Cite this article
Shinya Ikeno, Géza R. Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi and Tetsuya Haruyama, Protein Dot Stamp Using Hydrophobin as Immobilization Carrier, Sens. Mater., Vol. 16, No. 8, 2004, p. 413-420.



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