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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
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MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 16, Number 8 (2004)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 377-382
S&M574 Research Paper of Special Issue Published: 2004 Urease-Based Heavy Metal Ion Sensing Using a Silicon Transducer [PDF] Atsushi Seki and Satoshi Nomura (Received June 18, 2004; Accepted November 11, 2004) Keywords: light-addressable potentiometric sensor (LAPS), urease, inhibition, heavy metal ion
A heavy metal ion sensor based on urease inhibition and a semiconductor transducer were fabricated and characterized. Urease (EC 3.5.1.5) was reacted with copper (II) ion and mercury (II) ion, and the activity of the enzyme was measured. The pH change resulting from the enzyme reaction was monitored using a light-addressable potentiometric sensor (LAPS). This device detected the alkalization of the solution due to the enzymatic reaction, and the alkalization depended on the heavy metal ion concentration. The alkalization rates were approximately linear from 10 M to 30 M and from 1 M to10 M for copper and mercury, respectively. In addition, urease was immobilized on the electrode surface. The sensor responded to the addition of copper, and the enzyme membrane was restored by treatment with EDTA.
Corresponding author: Atsushi Seki Cite this article Atsushi Seki and Satoshi Nomura, Urease-Based Heavy Metal Ion Sensing Using a Silicon Transducer, Sens. Mater., Vol. 16, No. 8, 2004, p. 377-382. |
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