Notice of retraction
Vol. 32, No. 8(2), S&M2292

ISSN (print) 0914-4935
ISSN (online) 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

Instructions to authors
English    日本語

Instructions for manuscript preparation
English    日本語

Template
English

Publisher
 MYU K.K.
 Sensors and Materials
 1-23-3-303 Sendagi,
 Bunkyo-ku, Tokyo 113-0022, Japan
 Tel: 81-3-3827-8549
 Fax: 81-3-3827-8547

MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.


MYU Research

(proofreading and recording)


MYU K.K.
(translation service)


The Art of Writing Scientific Papers

(How to write scientific papers)
(Japanese Only)

Sensors and Materials, Volume 14, Number 4 (2002)
Copyright(C) MYU K.K.
pp. 199-208
S&M482 Research Paper of Special Issue
Published: 2002

Real-Time Microchip Polymerase-Chain-Reaction System [PDF]

Yu-Cheng Lin, Min Li, Ming-Ta Chung, Ching-Yi Wu and Kung-Chia Young

Keywords: PCR chip, MEMS, HCV, Real-time detection

A real-time micro-polymerase chain reaction (μ-PCR) system was developed to monitor the amplification of the complementary DNA (cDNA) molecules of the hepatitis C virus (HCV). This system uses SYBR Green I dye as a fluorescent probe for real-time detection in the PCR process. The μ-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, and anodic bonding. Compared to the polypropylene tube used in conventional PCR equipment, the silicon reaction well, which has a high thermal conductivity, can improve the temperature uniformity of the sample and the desired temperature can be attained more rapidly. The closed-loop thermal cycling system consists of power supplies, a thermal generator, a computer-controlled PID controller, a data acquisition subsystem, and a fluorescent microscope. Fluorescence was monitored at the end of the 72℃ step of the PCR cycle at five-cycle intervals. This μ-PCR system enables in situ detection and achieves 35 thermal cycles in 26 min. Different initial concentrations of HCV cDN A were used and good performance of this μ -PCR system was verified. Using this real-time μ-PCR system, it is possible to achieve DNA amplification with real-time detection within 26 min.


Cite this article
Yu-Cheng Lin, Min Li, Ming-Ta Chung, Ching-Yi Wu and Kung-Chia Young, Real-Time Microchip Polymerase-Chain-Reaction System, Sens. Mater., Vol. 14, No. 4, 2002, p. 199-208.



Forthcoming Regular Issues


Forthcoming Special Issues

Special Issue on Sensors and Materials: Emerging Investigators in Japan
Guest editor, Tsuyoshi Minami (The University of Tokyo)


Special issue on Novel Materials and Sensing Technologies on Electronic and Mechanical Devices (2)-1
Guest editor, Teen-Hang Meen (National Formosa University), Wenbing Zhao (Cleveland State University), and Hsien-Wei Tseng (Longyan University)


Special Issue on Materials, Devices, Circuits, and Analytical Methods for Various Sensors (4)
Guest editor, Chien-Jung Huang (National University of Kaohsiung), Cheng-Hsing Hsu (National United University), Ja-Hao Chen (Feng Chia University), and Wei-Ling Hsu (Huaiyin Normal University)
Conference website


Special issue on Novel Materials and Sensing Technologies on Electronic and Mechanical Devices (2)-2
Guest editor, Teen-Hang Meen (National Formosa University), Wenbing Zhao (Cleveland State University), and Hsien-Wei Tseng (Longyan University)


Special Issue on New Trends in Robots and Their Applications
Guest editor, Ikuo Yamamoto (Nagasaki University)


Special Issue on Artificial Intelligence in Sensing Technologies and Systems
Guest editor, Prof. Lin Lin (Dalian University of Technology)
Call for paper


Copyright(C) MYU K.K. All Rights Reserved.