Sensors and Materials
Contents
 
  MYU
Sensors and Materials
1-23-3-303 Sendagi, Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3821-2930
Fax: 81-3-3827-8547
 




Sensors and Materials, Volume 29, Number 5 (2017)
Copyright(C) MYU K.K. All Rights Reserved.
pp. 567-573
S&M1350
http://dx.doi.org/10.18494/SAM.2017.1559
Published on May 10, 2017

Development of a Bead-Based Multiplexed Immunoassay Using Image Cytometric Analysis

Yoshiaki Ukita, Chiwa Kataoka, Kazuyuki Sawadaishi, Akinobu Yamaguchi, and Yuichi Utsumi

(Received December 13, 2016; accepted March 9, 2017)

Keywords: multiplexed immunoassay image cytometry, bead-based assay

In this paper, we present the demonstration of a size-encoded bead-based multiplexed immunoassay with image cytometric decoding and readout. Antibodies were immobilized on polystyrene microspheres by physical adsorption and employed for the demonstration of this duplexed immunoassay. First, we carried out a duplexed immunoassay with a mouse and rat IgG detection system and discussed the problem of cross-reactivity inherent to these immunoreaction systems. We then carried out a duplexed immunoassay with a mouse IgG and human albumin detection system, which was less cross-reactive. We subsequently demonstrated the application of size-encoded image cytometric decoding and the detection of mouse IgG and human albumin using this duplexed immunoassay system.

Corresponding author: Yoshiaki Ukita

[PDF]

SM1350

Copyright(C) MYU K.K. All Rights Reserved.